Mass Spectrometers
| Instrument | Ionization Mode | Technique(s) | Analytes |
| Varian Saturn 2200 | Electron ionization (EI) Chemical ionization (CI) | Gas chromatography/mass spectrometry (GC/MS) | Volatile organic molecules Complex mixtures |
| Agilent 6210 | Electrospray ionization (ESI) Atmospheric pressure chemical ionization (APCI) | Exact mass determination Liquid chromatography mass spectrometry | Organic and inorganic molecules Peptides and proteins |
| Applied Biosystems QSTAR | Electrospray ionization (ESI) Nanoelectrospray Atmospheric pressure chemical ionization
| Liquid chromatography mass spectrometry Multidimensional liquid chromatography (e.g. MudPIT) Peptide sequence tags De novo sequencing Isotopic labeling (iCAT, ITRAQ, etc.) | Peptides Proteins Oligonucleotides |
| Bruker Omniflex | Matrix-assisted laser desorption ionization (MALDI) | Intact mass Peptide mass map | Peptides Proteins Oligonucleotides Synthetic polymers Organics Some inorganics |
| Bruker Ultraflex Extreme | Matrix-assisted laser desorption ionization (MALDI) | Intact mass Peptide mass map Peptide sequence tag Top down sequencing Liquid chromatography mass spectrometry (off-line) Tissue imaging | Peptides Proteins Oligonucleotides Synthetic polymers Organics Some inorganics |
| Mascot Computer Cluster | N/A | Database search | Proteins |
Rates
Rates are hourly with a one hour minimum per sample (unless user run). The Varian Saturn and Bruker Omniflex are available to trained users for hourly work. Please contact Dr. Hasan for information on user training and certification.
| Instrument | Technique | LSU Campus | Outside LSU Academic | Industry |
| Varian Saturn 2200 | GCMS | $8 | $11 | $40 |
| GCMS (User Run) | 6 | NA | NA | |
| Bruker Omniflex | MALDI | 18 | 24 | 100 |
| MALDI (User Run) | 12 | NA | NA | |
| Agilent 6210 | ESI Accurate Mass | 15 | 20 | 100 |
| ESI AM (User Run) | 10 | NA | NA | |
| LCMS | 25 | 33 | 100 | |
| LCMS (User Run) | 20 | NA | NA | |
| Applied Biosystems QSTAR | ESI MS | 15 | 20 | 100 |
| Exact Mass | 50 | 60 | 100 | |
| Capillary LCMS | 25 | 60 | 100 | |
| Nano LCMS or MS/MS | 40 | 53 | 100 | |
| Nano LCMS (Own Column) | 25 | 33 | 100 | |
| 2D Gel MALDI and ESI MS/MS (per spot) | 40 | 53 | 100 | |
| 2D LC MS/MS (per step) | 30 | 100 | ||
| Proteomics Sample Prep | 25 | 33 | 100 | |
| Database Search | 20 | 27 | 100 | |
| oMALDI | 40 | 53 | 100 | |
| iTRAQ | 40+kit cost | 53+kit cost | 100+kit cost |
Faculty Contact
225-578-3417
Choppin 331
Dr. Kermit Murray is the primary faculty contact for the Mass Spectrometry Facility. He joined the LSU faculty in 2001 after seven years on the faculty of Emory University. Dr. Murray has more than 25 years of experience in mass spectrometry and ion chemistry. He received his Ph.D. from the University of Colorado and went on to post doctoral positions at Rice University and at Texas A&M University. Dr. Murray’s research is in the area of instrument development and applications of biological and environmental mass spectrometry.
Staff Scientists
Connie David
225-578-7079
Choppin 100
Connie David joined the facility in June, 2011. She has 25 years of experience in Mass Spectrometry encompassing environmental trace analysis, drug testing, protein analysis and metabolic product analysis with GC-MS and LC-MS. Prior to joining the facility she worked for 19 years at the LSU School of Veterinary Medicine where she worked in the Analytical Systems Laboratory / Equine Medication Surveillance Laboratory. During that time she worked with many graduate students on projects ranging from prostaglandin metabolism to protein analysis of various animal related systems. She began her mass spec career in the LSU Biochemistry Department with Dr. Roger Laine studying the mass spectrometry of carbohydrates and uterine glycoproteins.
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